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Fig. 1. Maternal Dkk1 is ubiquitously expressed. (A) Control
uninjected (uninj.) and sibling antisense oligo (AS1 5 ng)-injected oocytes
were frozen as mature oocytes, mid- (stage 8.5), early (stage 10.5) and late
gastrula (stage 12) stage oocytes, and assayed by real-time RT-PCR for the
relative expression of Dkk1 mRNA. (B) Batches of two whole and
four animal- or four vegetal-half uninjected stage 6 oocytes were assayed by
real-time RT-PCR for the relative expression of VegT and
Dkk1 mRNA. (C) Batches of two whole and four right-, left-,
dorsal- or ventral-half four-cell stage embryos were assayed by real-time
RT-PCR for the relative expression of Dkk1 mRNA. (D) Control
uninjected (uninj.) and sibling antisense oligo (AS1 5 ng, 10 ng)-injected
oocytes were frozen after 48 hours in culture as mature oocytes and assayed
for maternal Dkk1 mRNA expression.