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Fig. 5. Interactions between Wnt11 and Wnt5a. (A) Wnt11-HA
or Xnr2-HA mRNA (1 ng) was injected either alone or together with
Wnt5a-Myc mRNA (1 ng) in the same cells at the two-cell stage.
Immunoprecipitation was performed with anti-Myc antibody.
Co-immunoprecipitation shows Xnr2-HA present in the lysate (lanes 1-2) but
absent in the anti-Myc IP-complex (lanes 5-6); Wnt11-HA was present in the
whole lysates (lanes 3-4) and precipitated with Wnt5a-Myc (lanes 7-8).
Arrowhead, IgG band; asterisk, Wnt11-HA band. (B) Schematic description
of same cells (SC) and different cells (DC) injected with tagged Wnt11 and
Wnt5a mRNAs at the four-cell stage. (C) Co-immunoprecipitation shows
Wnt11-HA present in the whole lysates (lanes 1-2) and precipitated with
anti-Myc from both SC and DC injections (lanes 3-4). Wnt5a-HA is detected in
whole lysates (lanes 5-6) and after anti-GFP co-IP from SC and from DC
injections (lanes 7-8). (D) Schematic description of the TOPflash
reporter assay after Wnt mRNA overexpression in the oocyte. (E)
TOPflash Luciferase activity measure in blastulae derived from control
(uninj), Wnt5a-injected (Wn5a 5 pg), Wnt11 injected (Wnt11 5 and 10 pg) or
co-injected (Wnt5a 2.5 pg+Wnt11 5 pg) oocytes.
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