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Figure 1


Fig. 1. Normal ovarian differentiation requires GATA4-FOG2 Interaction. (A-K) Whole-mount in situ hybridization (ISH) was performed with the indicated RNA probes on XX gonads from wild-type, Gata4ki/ki and Fog2-/- E12.5-13.5 mouse embryos. In all images, samples are oriented with anterior towards the right, and a gonad (g) on top of a mesonephros (m) as indicated in A. In images in which gonadal expression is not apparent, a black dashed line is used to show the gonad-mesonephric border. (L) Real-time PCR analysis of Fst and Bmp2 gene expression in wild-type, Fog2-/- and Gata4ki/ki E12.5 XX gonads. The y-axis shows values for both genes normalized to Gapdh RNA copy number.





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