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Fig. 2. cyp26b1 is expressed in condensing chondrocytes and
perichondrium. Stainings of wild-type zebrafish at the stages indicated in
the upper right corners and with the in situ RNA probes or antibodies
indicated in the lower right corners. fli1a-positive neural crest
cells in D were visualized by anti-GFP immunostaining of a
Tg(fli1a:EGFP)y1 transgenic embryo
(Isogai et al., 2003).
(A,B,D,E,H,I) Lateral views; (C,L-Q) dorsal views; (F,J) longitudinal
sections; (G,K) transverse sections. (A-D) cyp26b1 is
expressed close to, but not within, postmigratory cranial neural crest (CNC)
cells. Arrows in A,B point to dlx2a-positive,
cyp26b1-negative cells that according to lineage-tracing data are
likely to give rise to the ethmoid plate (e). Arrows in C,D point to two
cyp26b1 domains close to the pharyngeal arch-forming CNC.
(E-H) cyp26b1 is expressed in chondrogenic mesenchymal
condensations. (F,G,H) Magnifications of regions indicated in E. In F,G,
pharyngeal endoderm is counterstained with zn5 antibody; in H, neural crest
derivatives are stained for sox9a transcripts; double-positive
domains in pharyngeal arch (pa) condensations are marked. (I-K)
cyp26b1 is expressed in perichondrial cells around the pharyngeal
arches (J), the ethmoid plate (K), and along the entire length of the
ceratohyal (ch) and the ceratobranchials (cb) (I; inset shows magnification of
one cb). (L-Q) By contrast, osx (L), opn (M) and
col10a1 (N) are restricted to perichondrial cells around the
ossifying, Alizarin Red (alR)-positive region of the ceratohyal (O). In
col10a1-positive cells, cyp26b1 levels are lower than in the
adjacent col10a1-negative perichondrium (P,Q; arrows point to cells
double positive for cyp26b1 and col10a1). spt, subpallial
telencephalon; vt, ventral thalamus.
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