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Fig. 4. cyp26b1 mutants and morphants display deficiencies in midline
cartilages of the neurocranium and visceral skeleton. All panels show
ventral views of zebrafish head regions. (A-D) col2a1 in situ
hybridization at indicated stages. (E-N) Alcian Blue stainings of
cartilaginous craniofacial elements at 120 hpf. Pharyngeal arches are numbered
(1, mandibulare; 2, hyoid; 3-7, branchial/gill arches 1-5). (E-G) Overviews of
visceral skeleton. (H-K) Magnified views of ceratohyals (H,I) or pharyngeal
arches 4-6 (J,K). Arrows in H,I point to ceratohyal (ch) attachment in
midline. (L-N) Flat-mounts of neurocranium, revealing the absence of medial
ethmoid (e) and anterior basicranial commissure (abc) in mutant and morphant.
anc, chondrocytes of anterior neurocranium; bb, basibranchial; bh, basihyal;
cb, ceratobranchials; m, Meckel's cartilage; n, notochord; pq, palatoquadrate;
t, trabeculae cranii.
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