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Fig. 5. cyp26b1 mutants and morphants display increased ossification of
endochondral and intramembranous craniofacial bones. (A-C) Lateral
views and (D-F) ventral views of zebrafish larval heads after staining
of ossified matrix with Alizarin Red at indicated ages. Insets in A-C show
hyomandibula (hm; dorsal element of arch 2) of larvae of same genotype stained
with Alcian Blue at 120 hpf. Mutant and morphant show an opercle (op) of
increased size, whereas the hyomandibula fails to ossify, although its
cartilage model is properly formed (insets). A similar combination of gain of
opercle and loss of hyomandibula ossification has previously been described
for endothelin mutants (Kimmel et
al., 2003), possibly reflecting a morphogenetic effect of
signaling to pattern ossification along the dorsoventral axis of the second
arch and its associated elements. (D-F) Endochondral ossification within the
ceratohyal (ch) is much more advanced in the mutant (E), comparable to the
situation in a wild-type sibling 2 days later (F).
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