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Fig. 5. Inhibition of canonical Wnt signaling by sclerostin in Bmpr1a
cKO mice. (A) QRT-PCR analysis for Sost, Dkk1, Dkk2 and
Lrp5 using calvariae at E16.5, E17.5 and E18.5. Values are expressed
relative to wild type at E16.5. Student's t-test;
*P<0.05. (B) QRT-PCR analysis for Wnt target
gene Axin2 and Ctgf using E18.5 calvariae. Expression levels
of Axin2 and Ctgf were significantly increased in cKO
calvariae. Student's t-test; *P<0.05.
(C) Immunohistochemical staining of sclerostin (brown) counterstained
with Hematoxylin (blue) using E17.5 calvariae. Broken line, osteoblasts. Scale
bars: 50 µm, left panel; 10 µm, right panel. (D) Detection of
BMPR1A (green), canonical Wnt signaling (blue) and sclerostin (red) using
E17.5 calvariae. Canonical Wnt signaling was assessed by β-gal staining
using TOPGAL mice. Nuclei were stained with DAPI (blue). Broken line,
osteoblasts. Scale bars: 20 µm. (E) Immunohistochemical staining for
sclerostin (red) in primary osteoblasts from Bmpr1a cKO and wild-type
control. Nuclei were stained with DAPI (blue). Scale bars: 20 µm.
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