DEV029009 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Sktl is required for the maintenance of oocyte polarity during early oogenesis. WT (B,D,F,H) and sktl^M5-null mutant (A,C,E,G,I) germline clones marked by the loss of nuclear-GFP (green). (A-C) Egg chambers stained for DNA (blue in A, white in B,C). The mutant egg chambers fail to grow and remain at stage 2. Wild-type (B) and sktl^M5 mutant (C) egg chambers at stage 2. In both cases, the DNA of the oocyte is compacted into a karyosome (arrowheads in A-C), whereas the nurse cells are polyploid. (D,E) WT (D) and skt1 mutant (E) germarium stained for the synaptonemal complex (SC, red). In mutant cysts, the SC becomes restricted as normal to one cell in region 3, and is maintained in the oocyte at stage 2 (arrows), indicating that meiosis is correctly maintained only in the oocyte. (F) WT germarium stained for γ-Tubulin (red) to label the centrosomes. The centrosomes first accumulate in the oocyte before switching to the posterior in region 3 (arrows). (G) Chimeric germarium containing sktl mutant germline cysts. The centrosomes still localize to one cell in the mutant cyst and translocate normally at the posterior of the oocyte (arrow). (H) Wild-type germarium expressing nuclear GFP (green) and stained for Orb protein (red). Orb moves from the anterior to the posterior of the oocyte in region 3. (I) Chimeric germarium containing sktl mutant germline cysts. In the mutant cysts, Orb (red) accumulates in one cell in region 3 and starts to translocate to the posterior cortex (arrow). This enrichment at the posterior disappears during stage 2 of oogenesis (arrow). In older oocytes, Orb is found diffusely throughout the entire cyst.

• Supplemental Figure S2 -

  Fig. S2. Oskar is not translated in sktl mutant oocytes. Stage 10 Drosophila egg chambers stained for Osk (red) and with DAPI (blue). (A) Osk is localized to the posterior in the WT. (B) In sktl^2.3 mutants, Osk is not translated.