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Figure 7


Fig. 7. Sktl activity is required for Moesin phosphorylation. (A,B) Immunostaining using an antibody specific for the phosphorylated form of Moesin (P-Moe). P-Moe staining is found at the cortex in WT Drosophila oocytes (red in A) and is abolished in sktl2.3 mutant germline clones marked by the loss of GFP (B). (C,D) Western blot of ovary extracts (1, 2 and 4 mean that the equivalent of 1, 2 and 4 ovaries were loaded) from WT and sktl2.3/sktl{Delta}5 probed with anti-P-Moe (C), anti-Moesin (D) and anti-Tubulin ({alpha}-tub) as a loading control (C,D). (E,F) Immunostaining using an antibody against Moesin. Moesin is localized at the cortex in both WT and sktl2.3/sktl{Delta}5 oocytes (E,F, arrowheads). Asterisk, nucleus.





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