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Files in this Data Supplement:
Fig. S1. Dscam and netrin phenotypes in Bolwig's nerve. The axons of Bolwig's nerve (BN; the larval photoreceptor organ) in stage 17 embryos were visualized with monoclonal antibody 22c10. Staining of additional axons and cell bodies of the peripheral and central nervous systems are visible. (A) Wild-type BN showing tightly bundled axons and growth cones (arrow) as they grow around the brain hemisphere making a characteristic turn (arrow). (B) Dscam homozygous embryo showing defasciculation of BN and refasciculation to form a small 'loop' (arrow). (C) NetA,B hemizygote showing extensive defasciculation of BN and refasciculation to form a large loop (arrow). (D) fra3 homozygote showing defasciculation (arrow) and separation of a growth cone from a club-like structure (arrowhead). (E) Dscam fra/+ + trans-heterozygous embryo showing defasciculation (arrow) and a club like structure (arrowhead). (F) NetA,B/+; Dscam/+ trans-heterozygote showing a large loop (arrow).
Fig. S2. Axon guidance defects in abl mutants. Ventral nerve cords from stage 16 embryos stained with monoclonal antibody BP102 to visualize CNS axons. (A) Wild-type embryo. (B) Embryo lacking both maternal and zygotic abl. Note the lack of commissural axons and thinned longitudinal tracts suggestive of axon growth arrest. (C) Embryo lacking fra and zygotic abl activity.
Fig. S3. Salivary glands defects in Dscam combination mutants. Salivary glands in stage 13/14 embryos were visualized with a forkhead gene in situ hybridization probe. Each column is one genotype as indicated at the top. (1) Wild type. Dorsal view of a wild-type embryo showing the bilaterally symmetrical salivary glands (arrows), cells of the stomatogastric nervous system that also label with forkhead (arrowhead) and the midgut (asterisk). The side views show the salivary glands describing a gentle curve. (2) Dscam mutant embryo showing salivary glands that are slightly shorter than usual. The origin of the shortening is seen in the left-hand side view in which the increased curvature of the gland (arrow) is obvious. (3) Dscam fra double mutant embryo with one gland dramatically shorter than wild type. The left-hand side view shows the shorter gland length to be due to a kinked pattern of growth and partial growth arrest.
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