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Fig. 4. Dll1 overexpression induces neuronal differentiation in a
non-cell-autonomous manner. (A) Primary NPCs were prepared from
3-day sphere cultures (3 DIV) of the neocortical cells of E12.5 ICR mice,
infected with a retrovirus encoding GFP (pMX-GFP, control), or both GFP and
Dll1 (pMX-Dll1-IG), at a high titer and analyzed as in
Fig. 3C. (B) Primary
NPCs were prepared from 3-day sphere cultures (3 DIV) of the neocortical cells
of E12.5 ICR mice and plated at different cell densities (0.26x,
0.52x and 1.04x105 cells/cm2). These cells
were infected with a retrovirus encoding GFP (pMX-GFP, control), or both GFP
and Dll1 (pMX-Dll1-IG), at a low titer and analyzed as in
Fig. 3B (clonal analysis).
*P<0.05. (C) Primary NPCs were prepared from
3-day sphere cultures (3 DIV) of the neocortical cells of E12.5 ICR mice and
infected with a retrovirus encoding GFP (pMX-GFP, control), or both GFP and
the delta-like 1 intracellular domain (pMX-DICD-IG), at a low titer and
analyzed as in Fig. 3B (clonal
analysis).
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