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Fig. 5. Dll1 overexpression in a small number of NPCs in the developing mouse
neocortex induces neuronal differentiation. Retroviruses encoding GFP
alone (pMX-GFP, control), or both GFP and Dll1 (pMX-Dll1-IG), were
injected into the lateral ventricle of ICR mice at E12.5. Under these
conditions, only a small proportion of cortical NPCs were infected. Brains
were excised 2 days later and examined. (A,B) The location of
the GFP-positive cells was determined by immunohistochemistry with anti-GFP
and TO-PRO-3 (nuclear staining). Typical results are shown. (C)
Quantification of the distribution of GFP-positive cells in the neocortex.
Data are the mean±s.e.m. of values from eight sections of each brain
compared between littermates. Similar results were obtained from seven
independent littermates. (D) Brain sections were stained with anti-GFP
and TuJ1, and the percentage of TuJ1-positive cells among GFP-positive cells
in the whole neocortex was determined. Data are the mean±s.e.m. of
values from eight sections of each brain compared between littermates. Similar
results were obtained from three independent brains.
*P<0.01, **P<0.0001. VZ,
ventricular zone; SVZ, subventricular zone; IZ, intermediate zone; CP,
cortical plate. Scale bars: 200 µm.
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