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Figure 1


Fig. 1. Quiescence of NB3-3T and proliferation of NB3-3. NB3-3 (arrowhead) and its progeny were visualized by eagle-GAL4>UAS-mCD8GFP (eg>GFP; green) or eagle-GAL4>UAS-LacZ (eg>LacZ; green). Drosophila NBs were labeled using anti-Miranda (Mir; white). (A-C) Embryos were pulse-labeled with BrdU (magenta) at the indicated time (AEL, after egg laying). (A) Thoracic NB3-3 (NB3-3T) and (B) abdominal NB3-3 (NB3-3A). (C) BrdU-positive NB3-3 cells were counted. n, number of NB3-3 scored. (D) Wild-type larvae were continuously labeled with BrdU (magenta) from hatching until the indicated time (ALH, after larval hatching). (E) NB3-3T (left) and NB2-4T (right) from the same hemisegment of embryo pulse-labeled with BrdU at stage 15. When NB3-3T became elongated, its neighboring NB2-4T remained round and incorporated BrdU (magenta). The shape and BrdU incorporation of NB3-3 are represented diagrammatically beneath A,B,D.





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