|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Temporal transcription factors regulate the timing of entry to
quiescence. (A-X) Drosophila embryos were labeled for the
indicated markers. Genotypes are shown at the top. NB3-3T was identified by
eg>GFP or Eg (green). (A-H) Thoracic NBs (A-D) and NB3-3T
(E-H) at stage 16. In wild-type (A,E), pdm (C,G) and pdm cas
(D,H) embryos, NBs become elongated, whereas cas mutant NBs remain
round (B,F). (I-L') NB3-3T (white arrowhead) in embryos pulse
labeled with BrdU (I-L, magenta, I'-L',white) at the indicated
stages. In pdm (K,K') and pdm cas (L,L')
embryos, BrdU incorporation is only occasionally observed in NB3-3T at stage
13. In cas embryos (J,J'), BrdU incorporation is still observed
at stage 16. (M-T) Nab expression (magenta) in NB3-3T. (U-X)
Thoracic EL neurons at stage 16 or 17. In cas embryos (V), the number
of EL neurons increases and Nab expression is not detected, whereas in
pdm (W) and pdm cas (X) embryos, the number of EL neurons
decreases and Nab-positive EL neurons are observed precociously. Bottom:
phenotype summaries. (Y) The number of BrdU-positive NB3-3T in
pdm embryos. n, number of NB3-3T scored. (Z) Schematic model
of formation of thoracic EL neurons.
|
