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Fig. 6. Nab and Sqz regulate temporal state and NB quiescence. (A) BrdU incorporation (magenta) in NB3-3T (white, visualized by Mir) of nab and sqz⁰²¹⁰² Drosophila embryos pulse-labeled at the indicated times. NB3-3 lineage is visualized by Eg (green). (B) BrdU incorporation (magenta) in the thoracic hemisegment of wild-type, nab and sqz⁰²¹⁰² newly hatched larva (0-6 hours ALH). (C,D) Cas expression (magenta) in NB3-3T (C) and Cas and Svp expression (magenta) in NB3-3A (D) from embryos. Svp expression is not detected in NB3-3T (data not shown). Genotypes are shown on the left. The NB3-3 lineage is visualized by eg>GFP or Eg (green). Arrowhead indicates NB3-3. (E) Phenotype summaries. In sqz^ie nab double mutants, NB quiescence was inhibited as in nab mutants (data not shown). (F) NB3-3T (visualized by Mir), BrdU incorporation, Pdm expression and Grh expression in thoracic NBs of wild-type, cas and wor>nab sqz in cas embryos at stage 16. The co-expression of Nab and Sqz rescues the defect in Grh expression, indicating that NBs maintain stem cell characteristics and undergo temporal change.

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