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Figure 8


Fig. 8. Ror2 mediates chemotactic action of Wnt5a in the developing palate. (A-A'') BSA bead fails to exert chemotactic effect on DiI-labeled mesenchymal cells and grafted Egfp-expressing cells (insert in A'). (B-B'') Wnt5a acts as a potent chemoattractant to attract DiI-labeled mesenchymal cells and grafted Egfp-expressing cells (inserts in B', 48 hours in culture). Arrows indicate DiI-labeled cells attracted to Wnt5a bead. (C-C'') Few DiI-labeled cells (arrows) are attracted to Wnt5a bead in an explanted palatal shelf from Ror2-/- embryo. (D-D'') Fgf10 acts as a chemoattractant for palatal mesenchymal cells. Heparin beads were used for Fgf10 soaking, which is invisible under UV light, and are outlined by the broken line. B, bead.





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