DEV025080 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Hypotrophy of the P21 Dicer-ablated cortex. Nissl staining of 50-µm coronal Vibratome sections through the rostral cortex (Cx) of control (Emx1^Cre/wt Dicer^flox/wt, left) and conditional Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox, right) P21 littermate mice. Asterisks indicate the lumen of the ventricle. Scale bar: 100 µm.

• Supplemental Figure S2 -

  Fig. S2. Reduced postnatal growth of mice with Dicer-ablated cortex. Wild-type (Emx1^wt/wt Dicer^flox/wt or Emx1^wt/wt Dicer^flox/flox, triangles), conditional heterozygous (Emx1^Cre/wt Dicer^flox/wt, squares) and conditional homozygous Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox, diamonds) littermate mice from four independent litters were weighed at the indicated days after birth; weaning was at P21 and genotyping at P24-25. Data are the mean of 14 (wild-type, eight females, six males), 12 (heterozygous, five females, seven males) and three (Dicer KO, two females, one male) mice; bars indicate s.d.

• Supplemental Figure S3 -

  Fig. S3. Lack of interneurons and defective cortical connections in P7 Dicer-ablated cortex. Double immunofluorescence confocal microscopy (6-µm single optical sections) of a 50-µm coronal Vibratome section through the rostral cortex (Cx) of control (Emx1^Cre/wt Dicer^flox/wt, left) and conditional Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox, right) P7 littermate mice, stained for calretinin (top) and neurofilament (bottom). Calretinin-positive interneurons and their neurites are indicated by arrows and arrowheads, respectively; asterisks indicate the ventricular lumen. Scale bar: 200 µm.