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Fig. 1. Conditional ablation of Dicer in neural progenitors of the dorsal telencephalon during mouse embryonic development results in a smaller cortex. (A) Intrinsic EGFP fluorescence (white) with (a) and without (b) DAPI staining (blue) in a 10-µm coronal cryosection through the brain of an E13.5 mouse embryo obtained from crossing an Emx1^Cre/wt mouse with a Z/EG reporter mouse. Note the specific expression of EGFP in the dorsal telecephalon. Mb, midbrain. The dashed box in panel a indicates the region shown at higher magnification in panel b; this region was chosen for subsequent analyses of high-magnification images (20x objective). (B-E) In situ hybridization on 10-µm cryosections through the heads of E10.5-12.5 control (Emx1^Cre/wt Dicer^flox/wt) and conditional Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox) littermate embryos, using LNA antisense probes for miR-9 (B,C) and miR-124 (D,E). (B,D) Low-magnification overviews. Brackets indicate the region of the dorsal telencephalon used for subsequent analyses; dotted lines and arrows indicate the ventral telencephalon that is unaffected by Emx1-Cre-mediated Dicer ablation. (C,E) Higher magnification of the E10.5 (E) and E11.5 (C) mesencephalon and of the E10.5-12.5 dorsal telencephalon. Note the lack of accumulation of mature miR-9 (B,C) and miR-124 (D,E) specifically in the dorsal telencephalon upon Dicer ablation. VZ, ventricular zone; BL, basal lamina; brackets in C,E indicate the pre-plate (E11.5) and cortical plate (E12.5). (F) Comparison of P0 brains of control (Emx1^Cre/wt Dicer^flox/wt, left) and conditional Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox, right) littermate mice. (Top) Dissected brains. Note the reduced size of the cerebral cortex (Cx) and olfactory bulbs (OB) in the Dicer KO brains. Dashed lines indicate the location of the coronal cryosections shown in F' and F''. (F',F'') DAPI staining of 10 µm coronal cryosections. Note the reduced size of the cortex (Cx) and hippocampus (Hp) (which lacks its typical structure), but not midbrain (Mb), in the Dicer KO brains. Scale bars: 500 µm in F',F''; 250 µm in Aa,B,D; 100 µm in Ab; 50 µm in C,E.

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