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Fig. 6. Dicer ablation in the embryonic dorsal telencephalon results in reduced upper layer neurons, but not deep layer neurons, in the postnatal cortex. (A-F) Control (Emx1^Cre/wt Dicer^flox/wt; B,E) and conditional Dicer knockout (Dicer KO, Emx1^Cre/wt Dicer^flox/flox; C,F) mouse embryos were subjected to BrdU labeling in utero either at E12.5 plus E13.5 (B,C) or at E17.5 plus E18.5 (E,F), and the respective littermate pups were analyzed at P1, as outlined in A,D. (B,C,E,F) Triple immunofluorescence confocal microscopy (3-µm single optical sections) of 10-µm coronal cryosections through the caudal cortex stained for BrdU (red), Tbr1 (green) and Brn1 (green); DAPI staining in blue. Ventricular surface (VS), layers VI and V-I, and the pial surface (PS) are indicated on the left. Scale bar: 50 µm. (G,H) Quantification of Tbr1-positive (G) and Brn1-positive (H) nuclei (immunostained as in B,C,E,F) in the cortical wall (excluding the VZ; see the densely packed Brn1-positive layer in the control cortex at the bottom of the corresponding panel), each expressed as percentage of DAPI-stained nuclei. Data are the mean of three fields from independent experiments; one third of two of these fields is shown in B,E, and in C,F. Bars indicate s.d. (I,J) Quantification of BrdU-labeled (BrdU+) nuclei (immunostained as in B,C,E,F) in the cortical wall (defined as in G,H). Data are for the field of which one third is shown in B,C (I), and in E,F (J). BrdU-labeled nuclei were scored for immunoreactivity with Tbr1 (black), Brn1 (gray), or neither (white).

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