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Fig. 8. Production of high-quality cloned embryos and offspring. (A)
Expression of Oct4 and Cdx2 in expanded mouse blastocysts derived from
normally fertilized embryos, from embryos produced by nuclear transfer with
intact cumulus and from embryos produced by nuclear transfer with cumulus
cells treated with GV oocyte cytoplasmic lysates. The classification is based
on the numbers of ICM blastomeres expressing immunoreactivity for Oct4. Type
I, more than 15 positive cells; type II, 10-15 positive cells; type III, fewer
than 10 positive cells. Oct4 is stained green and Cdx2 is red. Scale bar: 20
µm. (B) Proportions of blastocysts classified into these three
types. (C) Cloned mice were born from enucleated MII oocytes injected
with cumulus nuclei treated with cytoplasmic lysates of GV oocytes.
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