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Figure 2


Fig. 2. miR-126{Delta}/{Delta}, but not Egfl7{Delta}/{Delta}, mice exhibit incompletely penetrant embryonic lethality, edema and vascular leakage. (A,B) Breeding tables from heterozygous intercrosses show that Egfl7{Delta}/{Delta} mice are born at normal Mendelian ratios ({chi}2=0.741), whereas miR-126{Delta}/{Delta} embryos exhibit ~50% embryonic/perinatal lethality ({chi}2<0.001). Numbers in parenthesis indicate embryos found with edema. Red numbers indicate deviation from Mendelian ratios. (C) Wild-type (wt) and Egfl7{Delta}/{Delta} embryos were phenotypically indistinguishable, whereas 50% of the miR-126{Delta}/{Delta} embryos displayed subcutaneous edema (*) at E14.5. (D) Hematoxylin and Eosin staining reveals the severity of the edema (*) in E14.5 embryos. (E) Varying degrees of subcutaneous hemorrhage are detected in ~20% of E15.5 miR-126{Delta}/{Delta} embryos; a severely affected embryo is depicted. Histological analysis shows red blood cells extravasating from a representative ruptured, leaky vessel (arrow). (F) Tie2-Cre-mediated endothelial deletion of miR-126 phenocopies the miR-126{Delta}/{Delta} edema (*) phenotype.





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