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Figure 6


Fig. 6. PTK7 is necessary for the migration of transplanted neural crest cells. (A) Transplantation assay. Two-cell stage embryos are injected with MO and 100 pg GFP RNA. At early neurula stage, premigratory neural crest cells are transplanted into uninjected control embryos from which the endogenous neural crest had been removed. (B,C) Transplant injected with 10 ng control MO showing normal neural crest migration 5 hours (B) and 12 hours (C) after transplantation. (D) A transplant injected with 10 ng PTK7 MO does not migrate 5 hours after transplantation, but (E) shows some migrating cells 12 hours after transplantation. (F) Graph summarizing a total of four independent experiments (n=73) using grafts injected with 10 ng control MO (n=29), 10 ng PTK7 MO (n=29) or 10 ng PTK7 MO co-injected with 1 ng PTK7 RNA, lacking the MO-binding sites (n=15).





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