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Fig. 7. Neural-crest-specific expression of PTK7 and dsh constructs.
Two-cell stage embryos were injected in one blastomere with plasmids
containing a minimal slug promoter driving either the expression of
GFP (A,B), wild-type PTK7 (C,D) or 
kPTK7 (E,F). (A-F) Tadpole stage
embryos analyzed by twist in situ hybridization. The injected side is
presented in the right panel (B,D,F). (A,B) Embryo injected with
100 pg slug-GFP plasmid. (C,D) Embryo injected with 100
pg slug-PTK7 plasmid. (E,F) Embryo injected with 100 pg
slug-kPTK7. Arrowhead in F indicates the inhibition of neural
crest migration. (G) Graph summarizing the percentage of migrating
neural crest cells in five independent injection experiments. The number of
injected embryos is indicated on each column. (H,I) Embryo
injected with 50 pg slug-DEP. (J-O) Embryo injected
with 50 pg slug-PTK7 together with 50 pg slug-DEP
(J,K) or 50 pg slug-dsh (L,M) or 50 pg slug-PDZ
(N,O). (P) Graph summarizing three dsh and PTK7 co-injection
experiments. The number of injected embryos is indicated on each column.
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