|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Altered expression profile of bHLH transcription factors in the two
regions of Pax6 mutant RPCs. (A-J) At E15, the expression in
control (A-E) and Pax6flox/flox;
-Cre (F-J)
of Crx (A,F), Atoh4 (B,G), Atoh3 (C,H), Neurod1 (D,I) and Atoh7 (E,J) was
characterized on adjacent sections by fluorescent in situ hybridization
(green). On the same sections, Pax6 expression was determined by indirect
immunofluorescence analysis (red). In the control, Crx was detected in the
prospective photoreceptor layer (A), while in the
Pax6flox/flox;-Cre, Crx was upregulated in
the peripheral region of the Pax6-deficient OC (F, surrounded by broken lines
labeled 1), but was downregulated in the mutated RPCs that are located more
centrally (F, surrounded by broken lines labeled 2). inl, inner nuclear layer;
le, lens; nbl, neuroblast layer; nnp, non-neuronal progenitors; rpe, retinal
pigmented epithelium. Asterisk indicates cells that escaped the recombination.
Scale bar: 75 µm.
|
