DEV023572 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Chiasm cells were counted after 48 hours in culture and after immunohistochemistry. The chiasm cell density for each co-culture combination did not differ significantly. Numbers of cultures are indicated in parentheses.

• Supplemental Figure S2 -

  Fig. S2. (A) Image of a retinal explant labelled with antibodies against neurofilament (NF), which marks axons, and Brn3a, which marks RGCs, counterstained with TO-PRO-3. (B) Image shown in A in which the green channel was converted to black and white. A line was traced 45 µm from the edge of the explant to produce the yellow measuring polygon. (C) Magnification of neurite fascicle showing how the width was measured (white line). (D) If a neurite did not grow outwards perpendicular to the explant border, the neurite width was measured where the polygon first touched the neurite/fascicle (white line). Scale bars: 100 µm in A,B; 400 µm in C,D.

• Supplemental Figure S3 -

  Fig. S3. Numbers of Brn3a-positive RGCs for each retinal quadrant do not differ between wild-type and Foxg1^-/- embryos at (A) E14.5 or (B) E16.5.

• Supplemental Figure S4 -

  Fig. S4 (A) Mean percentage axon coverage (± s.e.m.) for all DN retina-chiasm co-cultures at distances of 23, 45, 68 and 91 µm from the retinal explant. (B) Summary graph of mean percentage axon coverage at varying distances from the retinal explant for all co-cultures. Abbreviations: DN, dorsonasal; VT, ventrotemporal; r, retina; oc, optic chiasm. Numbers of cultures are indicated in parentheses in B.