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Fig. 6. Loss of Foxg1 from retina or chiasm impairs outgrowth of dorsonasal
retinal axons on chiasm cells in culture. (A-D) Co-cultures of
Foxg1+/± or Foxg1-/- dorsonasal
(DN) retinal explants with dissociated Foxg1+/± or
Foxg1-/- chiasm cells; immunohistochemistry is for the
axonal marker neurofilament (NF; green) and the RGC marker Brn3a (red); the
nuclear counterstain TO-PRO-3 (blue) reveals cells in the retinal explant and
surrounding dissociated chiasm cells. Cultures in A and C are shown at half
the magnification of those in B and D. (A'-D') Brn3a
expression in co-cultures A-D, respectively. (E) Mean percentage axon
coverage (±s.e.m.) 45 µm from the retinal explant, showing
significant differences among the four combinations. (F) Mean lengths
of the five longest neurites (± s.e.m.). (E,F) Numbers of explants are
in parentheses. One-way ANOVA revealed significant differences in outgrowth
among retinal explants. Significant differences with P values are
indicated above each bracket. Scale bars: 200 µm in A,C; 100 µm in B,D;
10 µm in A'-D'.
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