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Fig. 4. The basement membrane undergoes early and ectopic breakdown in the inner
ear of Lrig3 mutant mice. (A,F) Transverse plastic
sections through inner ears of E12 Lrig3+/- (A) and
Lrig3-/- (F) littermates, with magnified views
of the boxed areas (A',F'). Dorsal is upwards; lateral is towards the right.
In controls, epithelial cells in the fusion plate intercalate to form a single
layer of cells (A'), but in mutants, this region is expanded (brackets,
F'). Scale bar: 50 µm. (B,C,G,H)
Immunofluorescent detection of collagen IV (B,G) and all laminins (C,H) in E12
Lrig3+/- (B,C) and Lrig3-/-
embryos (G,H) sectioned in the transverse plane. In homozygotes, the basal
lamina is disturbed by breaks in the laminin network and ectopic accumulation
of collagen IV (arrowheads, G,H). Scale bar: 50 µm.
(D,E,I,J) Electron micrographs of the regions
indicated in C and H. The basement membrane is continuous in heterozygotes
(arrowheads, D,E) but is absent (asterisks, I) or severely disrupted
(asterisk, J) in homozygotes. Scale bar: 500 nm.
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