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Fig. 1. Pcdh- genes are broadly expressed in the retina. (A-D) Retinas from Pcdh- ^fusg/fusg mice, expressing a Pcdh--GFP fusion protein, were stained with antibody to GFP (green in A) and DAPI (blue in A). Pcdh--GFP fusion proteins are concentrated in the process-rich outer plexiform layer (OPL), inner plexiform layer (IPL) and retinal fiber layer (arrow), as well as in outer segments (OS) of photoreceptors, outer and inner nuclear layers (ONL, INL) and the ganglion cell layer (GCL). (B-D) High-magnification images of areas labeled in A'. (E) In situ hybridization to P21 retina using a probe against the Pcdh- common intracellular domain. (F-K) Dissociated Pcdh- ^fusg/fusg retinal cells immunolabeled with cell-type-specific antibodies (red), anti-GFP (green) and DAPI (blue). Pcdh--GFP-positive cells are labeled with the photoreceptor (PR) marker recoverin, the horizontal cell (HZ) marker calbindin, the amacrine (AC) marker syntaxin, the RGC marker Brn3a, and the Müller Glia (MG) markers glutamine synthetase (H) and Sox9 (K). Scale bars: 50 µm in A,E; 10 µm in B-D.

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