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Fig. 3. Subcellular localization of Pcdh-
proteins in the synaptic
plexiform layers. (A-E) Confocal images of the OPL of
Pcdh- fusg/fusg retinas double labeled
with anti-GFP (green) and antibodies against proteins present in the synapses
that photoreceptors form on horizontal and bipolar cells (red). (A)
Bassoon, a component of synaptic ribbons in all photoreceptor nerve terminals.
(B) PSD-95, a component of rod terminals (spherules). (C) Peanut
agglutinin (PNA) labels cone terminals (pedicles). (D) PKC
a
component of rod bipolar dendrites (arrow). (E) Calbindin, a component
of horizontal cell processes (open arrow) that terminate onto rod spherules as
well as horizontal cell processes that stratify in the inner OPL (open
arrowhead). Pcdh- proteins are present in both spherules and pedicles,
and in bipolar and amacrine processes, but are not seen in horizontal cell
varicosities (open arrow). (F) A single confocal plane of the IPL of
Pcdh- fusg/fusg retinas labeled with
anti-GFP (green) and antibodies to PSD-95 (red), which marks excitatory
postsynaptic sites in this lamina. Fine Pcdh--GFP puncta are
distributed throughout the IPL, and are present at but not restricted to
synapses. Scale bars: 10 µm.
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