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Figure 7


Fig. 7. Sublamina-specific targeting of amacrine and bipolar processes in the IPL in the absence of Pcdh-{gamma} genes. (A,B) ChAT- (red) and vGluT3-positive amacrine subsets (green) in Chx10-Cre;Pcdh-{gamma} fcon3/+ (control) and Chx10-Cre;Pcdh-{gamma} fcon3/fcon3 retinas. ChAT-positive processes ramify in sublaminae (S) 2 and 4, and vGlut3-positive processes ramify in S3. (C,D) Synaptotagmin 2 (SytII) -positive OFF bipolar processes (red) and G{gamma}13-positive ON bipolar processes (green) ramify in the outer and inner regions of the IPL, respectively. In all cases, laminar specificity is retained in mutants, but marker-laminae are reduced and disrupted. (E) Sketch of IPL sublaminae stained by the markers used in this study. Scale bars: 50 µm.





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