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Figure 8


Fig. 8. Laminar specificity and synapse formation by Pcdh-{gamma}-deficient neurons rescued from apoptosis. (A-D) Sections from retinas mutant for Bax (Chx10-Cre;Pcdh-{gamma} fcon3/+ Bax-/-), Pcdh-{gamma} (Chx10-Cre;Pcdh-{gamma} fcon3/fcon3 Bax+/-), both (Chx10-Cre;Pcdh-{gamma} fcon3/fcon3 Bax-/-) or neither (Chx10-Cre;Pcdh-{gamma} +/fcon3 Bax+/-). Sections were stained with anti-bassoon (red) and Po-pro1 (blue). Thickness of IPL and INL are similar in Bax mutants and Bax, Pcdh-{gamma} double mutants; both are thicker than those in Pcdh-{gamma} mutants. (E-L) High power images of OPL (E,G,I,K) and IPL (F,H,J,L) from retinas in A-D. Density of synaptic puncta is similar in Bax mutants and Bax, Pcdh-{gamma} double mutants (see Fig. S2 in the supplementary material for quantification). (M,N) Chx10-Cre;Pcdh-{gamma} fcon3/fcon3 Bax-/- mutants immunostained for ChAT (red) and vGluT3 (green) (M) or synaptotagmin 2 (SytII, red), and G{gamma}13 (green) (N). All processes make lamina-specific arbors (compare with Fig. 7) and disruptions seen in Pcdh-{gamma} single mutants are absent in double mutants. Scale bars: 100 µm in A-D; 10 µm in E-L; 50 µM in M,N.





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