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Figure 5


Fig. 5. Head but not trunk ectoderm is competent to form olfactory placode. (a) HH8 hindbrain-level ectoderm expresses Dlx3 when grafted to presumptive nasal region (A-A''; arrowhead points to a Dlx3+/QCPN+ cell). (b) HH10 midbrain-level ectoderm expresses Pax6 when grafted to olfactory placode region (B-B''; arrowhead indicates an ingressing QCPN+ cell). (c-C'') Similar graft to b undergoes neuronal differentiation within olfactory pit (C-C'', arrowheads) and in ingressing cells lining olfactory nerve (C-C'', arrows). (d-D'') Grafts of HH8 trunk-level ectoderm fail to express Pax6. (e,E) Some head ectoderm grafts incorporate into nose, lens and cornea. (F) HH8 midbrain-level ectoderm expresses Pax6 in lens and cornea. (G) HH8+ hindbrain-level ectoderm is not competent to express Pax6 in cornea or olfactory placode (not shown), though three quail cells in the lens appear Pax6+ (arrowhead). la, lateral; m, medial; c, cornea; fb, forebrain; le, lens; op, olfactory placode; r, retina.





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