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Fig. 6. Olfactory progenitors are specified to form neurons by HH10; placodal
morphology is recapitulated in culture from HH13-14. (A-A'')
Dlx3 (red) and Pax6 (green) are robustly expressed in HH8 explants; 
1/3
cells co-express both (arrows, A'', yellow); non-overlapping Dlx3
(A,A'', arrowhead) or Pax6 (A',A'', arrowhead) domains appear
distinct. (B) HH10 explant with neuronal differentiation; most neurons
are Dlx3+/Hu+ (arrow), though some are Dlx3- (arrowhead). Insets show
high-magnification views. (C) In HH10 explants, most cells are Pax6+
and few are Hu+ neurons. (C') Arrow indicates cluster of
Dlx3+/Pax6+ cells. Dlx3+ and Pax6+ cells segregate from double-positive cells.
(D) Olfactory placode explanted at HH13 undergoes thickening in culture
and has some Dlx3+/Hu+ (red/blue) cells (arrows, insets). Pax7 (blue,
distinguishable from Hu by nuclear localization) delineates nasal mesenchyme
underlying olfactory placode. (E) Newly thickened placodal ectoderm
shows robust neuronal differentiation (Hu+, blue); some neurons are Pax6+
(green; arrowheads; insets). (F) Location of olfactory precursors at
HH8, HH10 and HH14 based on fate maps.
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