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Figure 3


Fig. 3. Junctional PtdIns(3,4,5)P3 synthesis is due to E-cadherin signalling. (A) Junctional PtdIns(3,4,5)P3 synthesis in a 16-cell morula (left) is inhibited upon removal of extracellular Ca2+ (middle; note the decompaction of the embryo). Readmission of extracellular Ca2+ allowed for PtdIns(3,4,5)P3 re-synthesis at cell-cell contacts (right; note embryo re-compaction). (B) Two-cell embryo (from a low-density culture; top) and 16-cell embryo (bottom) cultured in the presence of ECCD-1. The anti-E-cadherin antibody prevented junctional PtdIns(3,4,5)P3 synthesis and embryo compaction. (C) PtdIns(3,4,5)P3 synthesis upon embryo aggregation. The arrowhead points to the new contact region between two aggregated two-cell embryos. PtdIns(3,4,5)P3 was monitored with GFP-PHGRP1 (A,B) or GFP-PHAkt (C). Confocal images are representative of at least 12 similar observations.





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