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Figure 4


Fig. 4. The genetic interaction between Lgl and aPKC shows that Lgl phosphorylation by aPKC can regulate oocyte polarity. (A,B) Stau was normally localized in the apkck06403 heterozygous mutant (A), but mislocalized in apkck06403 mutant germline clones (B); mutant clones were marked by the absence of nuclear GFP (green). (C,D) Loss of one copy of apkc exaggerated the Stau mislocalization phenotype stemming from Lgl overexpression. Stau localization in these backgrounds was quantified (D). (E) Stau is normally localized in aPKC-GFP overexpression. (F) Overexpression of aPKC-GFP can rescue the Stau mislocalization phenotype caused by the Lgl overexpression. (G) Stau is mislocalized in the Lgl-overexpressing egg chambers. (H) Stau localization in these backgrounds was quantified.





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