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Fig. 6. Lgl interacts with Par-1 and is required for Par-1 localization.
(A,B) Both the GFP-Par-1(N1S) (A) and GFP-Par-1(N1S)
K* (B) were enriched at the posterior at stage 10.
(C,C') The GFP-Par-1(N1S) K* lost the posterior
enrichment in lgl4 germline cells. Mutant germline clones
were marked by the absence of β-gal staining (red) in the nuclei of the
nurse cells. (D-D") GFP-Par-1(N1S) colocalized with Lgl and was
detected throughout the oocyte cortex in egg chambers with co-overexpression
of Lgl-3A and GFP-Par-1(N1S). Arrows indicate the strong GFP-Par-1 and
arrowheads indicate the weak GFP-Par-1. (E) Immunoblots of anti-Lgl or
anti-GFP immunoprecipitation from ovaries with GFP-Par-1 (N1S) overexpression
(mat-Gal4 driver) or the wild-type control
(mat-Gal4 driver only). aPKC and GFP-Par-1 (N1S) proteins
were precipitated by the anti-Lgl antibody from GFP-Par-1 (N1S)
overexpression, and aPKC and Lgl proteins were precipitated by an anti-GFP
antibody from GFP-Par-1 (N1S) overexpression. Input was one-fifth of the total
ovarian extract. In anti-Lgl immunoprecipitates, controls were beads (without
added antibody) and peptide blocks (+pep); in anti-GFP immunoprecipitates,
controls were beads (without added antibody) and ovarian extract from
wild-type flies.
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