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Fig. 3. Delayed differentiation of sympatho-adrenal precursor cells in
Insm1 mutant mice. The differentiation of the sympatho-adrenal
precursor cells in Insm1lacZ/+ and
Insm1lacZ/Insm1lacZ mice was assessed at the
indicated stages using immunohistochemical analysis or in situ hybridization.
(A-P) Analysis of E10.5 embryos. (A-D) Immunohistochemistry using
antibodies against β-galactosidase (red), (A,B) Phox2b (green) and (C,D)
Phox2a (green). (E-J) In situ hybridization using probes specific for
(E,F) Hand2, (G,H) Gata3 and (I,J) Ret. (K,L)
Immunohistochemistry using antibodies against β-galactosidase (red) and
Th (green). (M,N) In situ hybridization using a Dbh-specific
probe. (O,P) Immunohistochemistry using antibodies against
β-galactosidase (red) and Tuj1 (green). There is a pronounced reduction
in the number of sympatho-adrenal precursor cells that express Phox2a, Hand2,
Gata3, Ret, Th or Dbh at E10.5 in
Insm1lacZ/Insm1lacZ mice. (Q,R)
Analysis of E12.5 embryos by immunohistochemistry using antibodies against
β-galactosidase (red) and Th (green). (S) Quantification of
β-galactosidase+ sympatho-adrenal precursor cells that co-express Th in
Insm1lacZ/+ and
Insm1lacZ/Insm1lacZ mice at various
developmental stages. (T,U) Analysis of E12.5 embryos by
immunohistochemistry using antibodies against β-galactosidase (red) and
Phox2a (green). (V) Quantification of the β-galactosidase+ cells
in sympathetic ganglia that co-express Phox2a at various developmental stages
in Insm1lacZ/+ and
Insm1lacZ/Insm1lacZ mice. (W,X)
Analysis of E12.5 embryos by immunohistochemistry using antibodies against
β-galactosidase (red) and Tuj1 (green). (Y) Quantification of
β-galactosidase+ sympatho-adrenal precursor cells that co-express Tuj1 in
Insm1lacZ/+ and
Insm1lacZ/Insm1lacZ mice at various
developmental stages. Single or double asterisks in S,V,Y indicate
P<0.05 and P<0.01, respectively. Scale bars: 50 µm
in A; 100 µm in C,Q.
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