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Fig. 5. Reduced size of secondary sympathetic ganglia in Insm1 mutant
mice. Secondary sympathetic ganglia of Insm1lacZ/+ and
Insm1lacZ/Insm1lacZ mice (E18.5) were analyzed
by (A,B) X-gal staining and by (C-H)
immunohistochemistry. Antibodies used were directed against: (C,D) Tuj1 (red)
and Th (green), (E,F) Tuj1 (red) and NPY (green); (G,H) Phox2a (red) and Tlx3
(green). Arrowheads in A and B indicate superior cervical and stellate
ganglia. Scale bars: 500 µm in A; 100 µm in C.