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Fig. 1. Limb development phenotypes in Fgfr2^Msx2-Cre mouse embryos. (A-F) Comparison of limb phenotypes in wild-type control (WT) and Fgfr2^Msx2-Cre mouse embryos. (A,D) Skeletal preparations of E15.5 embryos. (B,E) Skeletal preparations of P0 forelimbs. (C,F) Gross appearance of limb buds at E12.5. Note that the hindlimb bud is never formed and only appears as a small bulge (arrow and dotted line) in Fgfr2^Msx2-Cre embryos. (G-J) Histology of the distal hindlimb buds at E10 (G,H) and E10.5 (I,J) showing the failure to form a multi-layered AER (arrow) in Fgfr2^Msx2-Cre embryos. (K-N) Histology of the distal forelimb buds at E10 (K,L) and E10.5 (M,N) showing initial formation of an AER (arrow) in Fgfr2^Msx2-Cre embryos at E10 and regression to a single-layered epithelium by E10.5. (O-R) Whole-mount in situ hybridization showing Fgf8 expression in the AER at E10.5 (O,P) and E11.5 (Q,R). Note that Fgf8 is never expressed in Fgfr2^Msx2-Cre hindlimbs (arrow) and is prematurely lost in Fgfr2^Msx2-Cre forelimbs, accompanied by distal deformities (dotted line). Data are representative of at least three embryos. Scale bar: in G for G-N, 20 µm.

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