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Fig. 4. Eomes-deficient epiblast exhibits normal Fgf8/Snail
expression but fails to downregulate E-cadherin. (A)
Scanning electron microscopy of transverse sections through E7.5 control and
Eomes mutant embryos shows the mesenchymal morphology of cells
accumulating at the primitive streak of mutant embryos. Eomes mutants
are devoid of a mesodermal cell layer. (B) Immunofluorescence staining
using an anti-E-cadherin antibody in E7.5 Eomes mutant embryos
reveals failure of E-cadherin downregulation at the PS stage. Whereas mesoderm
of wild-type embryos is devoid of E-cadherin (arrowhead), the distinctive
tissue mass in mutants retains E-cadherin. (C,D) Fgf8
and Snail are expressed at appropriate sites and with normal
intensity in EomesN/CA; Sox2.Cre embryos when
analysed by whole-mount in situ hybridisation (C) or in situ hybridisation on
sections (D). The mutant PS shows overlapping expression of E-cadherin and
Snail, whereas in wild-type controls the expression domains are
mutually exclusive. (C) The Fgf target Spry2 is widely expressed in
Eomes mutants. (E) Wild-type and Eomes mutant
primitive streak explant cultures show indistinguishable migration behaviour
and efficiently downregulate E-cadherin in migrating cells. Broken lines
indicate the border of E-cadherin-positive explants.
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