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Fig. 6. Activin and Activin-related (daw) genes are expressed in
developing larval optic lobes and required for normal optic lobe development
and correct photoreceptor axon targeting in Drosophila.
(A,B) In situ hybridization of wild-type yw
mid-third-instar larval brain lobes with antisense probes of
activin-β (A) and daw (B). The transcripts of
actβ are abundantly expressed in both larval optic lobe and the
central brain lobe, whereas daw gene is also expressed in the larval
optic lobe (arrow) in addition to its known expression in glial cells.
(C) A daw promoter-enhancer Gal4 transgene drives the
expression of a uasGFP reporter in glia cells (green and yellow) in the
developing larval brain lobes. Glial cells were stained by an anti-Repo
antibody (red). (D) The majority (95%) of homozygous
dawex32 wandering third-instar larvae do not show abnormal
optic lobe development or photoreceptor axon targeting defects. (E) A
minority (
5%) of daw32 homozygotes show optic lobe
and axon targeting defects reminiscent of babo mutants. (F) Optic lobes
developed normally and photoreceptor axons target correctly in
actβed80 mutant larvae. (G) Double
mutants of the genotype
dawex11:actβed80 exhibit altered
R7 and R8 growth cone bundling and morphology. (H) The
dawex32;actβed80 double
mutants showed significantly enhanced penetrance of the strong optic lobe
phenotype. Central bl, central brain lobe.
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