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Fig. 1. Early presence of pericytes in Matrigel vessels. Matrigel plugs were
removed for analysis after 9 days (A-C) or 5 days (D-I). In A-F,
endothelial cells were labeled for CD31 (green), while pericytes were labeled
for NG2 (magenta). In G-I, pericytes were labeled for both NG2 (magenta) and
PDGFβR (green). Cell nuclei were stained using DAPI (blue). At 5 days,
the vessel-like networks contain NG2+PDGFβR+
pericytes in the absence of CD31-positive endothelial cells. At 9 days, both
types of cells are present in the structures. All panels represent
z-stacks of confocal images. Scale bar: 40 µm.
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