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Fig. 2. Functional perfusion of FGF2-induced Matrigel vessels. Mice
containing Matrigel plugs were utilized on day 5 (A-D) and day 9
(E-H) for FITC-dextran (FITC-dx; green) perfusion to identify vessels
connected to the circulation. Sections prepared from the plugs were stained
for DAPI (blue), CD31 (red) and NG2 (magenta). At day 5, prior to the
appearance of CD31+ endothelial cells, FITC-dextran is not present
in the cellular networks. At day 9, FITC-dextran perfusion is apparent in
vessels containing both pericytes and endothelial cells. All panels represent
z-stacks of confocal images. Scale bars: 40 µm.
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