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Fig. 4. Flow cytometric analysis of NG2-positive, CD34-positive populations.
(A,B) Flow cytometry was used to examine NG2 and CD34 expression
by (A) cells recovered from dissociated 5-day Matrigel plugs and (B) cells in
total adult bone marrow. In A, 8.2% of the Matrigel population expressed NG2
alone, 6.6% expressed CD34 alone and 74.2% expressed both markers. In B, 5.3%
of the total bone marrow population expressed NG2 alone, 8.1% expressed CD34
alone and 1.7% expressed both markers. In control experiments with second
antibodies alone, fewer than 0.5% of cells were scored as positive.
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