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Figure 6


Fig. 6. Bone marrow origin of vascular cells. Matrigel plugs were established in irradiated mice that had been reconstituted using bone marrow cells from an EGFP+ donor. At day 5 (A-D) sections were analyzed by staining for NG2 (magenta), CD34 (red) and DAPI (blue). The EGFP marker (green) is seen in cells that co-express NG2 and CD34 (arrows). Also at day 5 (E-H), NG2 (magenta), F4/80 (red), EGFP (green) and DAPI (blue) labeling were compared. The EGFP label is apparent in both populations of macrophages: F4/80+NG2+ (arrows) and F4/80+NG2- (arrowheads). At day 9 (I-L), sections were analyzed for NG2 (magenta), CD31 (red), EGFP (green) and DAPI (blue). The EGFP marker is frequently found in NG2+ cells (arrowheads), and more rarely in CD31+ cells (arrows). All panels represent z-stacks of confocal images. Scale bars: 40 µm.





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