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Figure 1


Fig. 1. Components of the Jak/Stat pathway are expressed in the GSC niche. (A) Schematic of a Drosophila germarium. Terminal filament cells (TFCs), cap cells (CpCs), escort stem cells (ESCs) and escort cells (ECs) are of somatic origin. Germline stem cells (GSCs) are in close contact with CpCs and contain apical spectrosomes that evolve into `exclamation mark' figures in post-mitotic GSCs. Cystoblasts (CB) also contain spectrosomes, but these do not maintain an apical localisation. CBs undergo four incomplete rounds of division giving rise to cysts of two, four, eight and sixteen cells interconnected by branched fusomes. (B) Wild-type germarium double stained to visualise spectrosomes and fusomes (anti-Hts, red) and the Upd ligand (anti-Upd, green). The accumulation of Upd protein is clearly visible in TFCs and CpCs. In addition, a weaker, speckled distribution is present in more-posterior cells, possibly corresponding to ESCs and ECs, cell types in which the Jak/Stat pathway is active (Decotto and Spradling, 2005). (C) Stat92E06346 germarium double stained to visualise Hts (red) and β-gal (green). Stat92E directs lacZ expression in TFCs, CpCs, ESCs and ECs (see also Decotto and Spradling, 2005). (D) Detection of upd, upd2 and upd3 mRNAs by RT-PCR in ovaries from virgin females. The expression of the embryonic gene ftz was used as a control for the specificity of the ovarian cDNA library (lanes 1 and 2). The expression of hedgehog (hh, lane 3) provided a positive control. Lanes 4, 5 and 6 show that upd, upd2 and upd3 are expressed in the ovary. (E) Germarium from a bab1-Gal4/UASt-DsRed female stained with anti-Hts (red). bab1-Gal4 directs DsRed expression (green) in TFCs, CpCs, ESCs and ECs. (F) Germarium from a tub-Gal80ts/UASt-upd2; bab1-Gal4/+ female stained with anti-Hts to show the tumour of spectrosome-containing cells produced after bab1-Gal4-driven overexpression of Upd2 in adult germaria. Anterior is up in all figures unless otherwise stated. Open arrowheads, TFCs; white arrowheads, CpCs; arrows, ESCs and ECs; asterisks, GSCs. Scale bars: 10 µm.





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