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Fig. 2. Jak/Stat signalling is required in the Drosophila ovary for GSC
maintenance. (A,B) Wild-type (A) and
hop27/hop25 updYM55 (B) germaria
stained with anti-Hts to visualise the clear reduction in the number of GSCs
and developing cysts in the mutant condition. (C,D)
nanos- Gal4/UASt-Src:GFP (C) and
hop27/hop25;
nanos-Gal4/UASt-Src:GFP (D) germaria dissected 10 days after
eclosion (AE). They were double stained with anti-Hts (red) and anti-GFP
(green) to visualise spectrosomes and to outline the germline cells,
respectively. The spectrosome in C displays the typical `exclamation mark'
shape (de Cuevas and Spradling,
1998); the spectrosome in D has lost its apical anchoring while
still maintaining its connection with the cystoblast spectrosome and was
therefore classified as an anchorless GSC spectrosome. The small `scar' of
spectrosomal material left on the apical side, adjacent to the CpCs, suggests
that the GSC spectrosome has severed its apical connection prior to
accumulating basally. (E) Bar chart representing the mean number of
GSCs (±s.d.) per germarium in hop27/FM7 (control),
hop27/hop25 and
hop27/hop25 updYM55 germaria.
Ovaries were dissected 2, 10 and 25 days AE. Black triangles indicate a
statistically significant difference between the given experimental condition
and its control (Student's t-test, P<0.01). (F)
Bar chart showing the percentage of anchorless GSC figures in
hop27/FM7 (control),
hop27/hop25 and
hop27/hop25 updYM55 germaria
dissected 2, 10 and 25 days AE. The number of germaria analysed for each
experiment (n) shown in E,F is indicated in E. Asterisks, GSCs; CB,
cystoblast. The white dashed lines delineate GSC-CB pairs. Scale bars: 10
µm.
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