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Fig. 3. β-Tubulin-positive cells are abnormal in differentiated Sox2 mutant
cell cultures from adult mouse. (A) β-Tubulin
immunofluorescence of normal (left) and mutant (right) day 9-differentiated
cells. Bottom: DAPI. Many of the mutant poorly arborized, less intensely
stained cells are barely visible in this low-magnification image. (B)
Higher magnification of normal and mutant β-tubulin staining. In mutant,
the arrowhead indicates a cell with well-developed neuronal morphology and
long arborizations; arrows indicate abnormal cells with short processes and
often weak β-tubulin staining typical of the mutant. (C) Time
course of β-tubulin expression during differentiation. `Mut, well
developed' indicates cells with long arborizations (B, wt or arrowhead in
mutant); `mut, total': total β-tubulin-positive cells (including those
indicated by arrows in B, mut). The abnormal phenotype is already observed at
day 5, the earliest stage when significant numbers of β-tubulin-positive
cells appear.
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