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Fig. 6. Co-expression of neuronal and glial markers in individual cells in Sox2
mutant cultures. Double immunofluorescence (β-tubulin and GFAP) of
normal (wt) and mutant (mut) day 9-differentiated cells. Typical wild-type
neurons (β-tubulin positive) show extensive arborization, are closely
associated with glia (which are GFAP positive), and are GFAP negative (top
row). Rare cells with a very undifferentiated morphology are weakly positive
for both markers (top, arrowhead). In mutant, various arborized cells are
positive for both β-tubulin and GFAP (second row, arrowhead; third row,
two arborized cells). Well-developed astrocytes are GFAP positive, but
β-tubulin negative (arrows, rows 2, 4). In mutant, some intensely
β-tubulin stained cells with neuronal morphology are also present (fourth
row, arrowhead); these cells are GFAP-negative, as in wild type.
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