DEV016097 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables S1-2 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Pupal phenotypes of RNAi targeting of the TcBR-C isoform Z3, Z4 and Z5. (A-D) Larvae were injected with dsRNA during the first day of the seventh instar. Note shortening of the wings and legs in Z3 and Z4(RNAi) animals relative to the control pupa (A; compare black and white arrows, respectively). Black arrowheads (B-D) point to the position to which the wings normally extend. Scale bar in A: 1 mm for A-D.

• Supplemental Figure S2 -

  Fig. S2. Chrysopa perla BR-C expression. The mRNA is detected throughout larval stages (second instar not shown) and increases to high levels before cocoon spinning (the time when prepupal development begins). Total RNA isolated from whole larvae was treated with DNase and used for cDNA synthesis. For RT-PCR conditions see Table 1 in the main text. Numbers indicate age in days of the indicated stages. Cocoon spinning takes place after four days of the third (final) instar. Expression of rp49 served as a control.

• Supplemental Figure S3 -

  Fig. S3. Wing development in TcBR-C(RNAi) animals. (A,B) Untreated transgenic larvae of the pu11 enhancer-trap line showed normal progression of wing development. (C,D) When pu11 larvae were injected with the common-region TcBR-C dsRNA during early-final instar, the wing discs (arrows) grew but attained an abnormal shape (C) compared with the triangular discs in a control early prepupa (B). Arrested late prepupae (D) failed to elongate wings (arrows); however, the ommatidia of their compound eye developed (arrowhead).